ASI 540 Series HPLC Pump: advanced pump functionality for the chromatographer
The ASI 540 Series HPLC pump is designed with the chromatographer in mind. The combination of an intuitive touchscreen display, patented pump technology and a wide range of pump heads makes the 540 Series suitable for virtually any liquid chromatography application, from nanolitre flow analysis to preparative-scale flash chromatography. The pump is available as an isocratic model (Model 541) and as a gradient system (Model 540G), in stainless steel or biocompatible PEEK.
ASI has supplied OEM pump assemblies for proteomics LC-MS, process instrumentation, pharmaceutical production, supercritical fluid chromatography, preparative chromatography, biotech and SEC/GPC systems.
Pump head versions
The 540 Series is available in six pump head classes, each in stainless steel (SST) and biocompatible PEEK, and several also in a UHPLC version (HP):
Micro delivers 0,5 µL/min to 4 mL/min at a maximum of 7.000 psi (SST) or 4.000 psi (PEEK). The UHPLC version (Micro SST UPLC) supports pressures up to 15.000 psi.
Analytical delivers 1 µL/min to 10 mL/min at a maximum of 7.000 psi (SST) or 4.000 psi (PEEK). The UHPLC version supports up to 15.000 psi.
Semi-Prep delivers 10 µL/min to 40 mL/min at a maximum of 3.500 psi.
Prep delivers 10 µL/min to 125 mL/min at a maximum of 1.500 psi.
Flash Chrome delivers 50 µL/min to 300 mL/min at a maximum of 500 psi.
Patented floating pump seal
A key feature of the ASI 540 Series is the patented floating pump seal. The seal moves in alignment with the piston, reducing wear and considerably extending seal life compared with fixed seal designs. The cartridge seal construction makes replacement easy via the front panel, without tools or disassembling the pump. All user-serviceable parts are accessible via the front panel.
Self-priming pump head
The pump head is self-priming and has an integrated priming/purge function for quick solvent changes. The pump primes any solvent at any desired flow rate, without the need for external syringes or drain valves.
Solvent compressibility compensation
The user can choose from a library of 14 solvents for automatic compressibility compensation. This guarantees accurate flow delivery regardless of the compressibility of the solvent used, improving the reproducibility of chromatograms.
Performance
For gradient reproducibility, RSD values of less than 1,0% have been achieved at 20 µL/min. Linear and step gradient profiles are accurately reproducible at 50 µL/min. Pulsation is at most 1% ΔP/P at 1.500 psi.
OEM options
The 540 Series is fully available for OEM private label, in both large and small volumes. ASI supplies the pump as a complete unit (OEM Option 1: pump only) or as a pump module including hydraulic module and pump controller (OEM Option 2). Custom configurations to specification are standard. Communication via Modbus RTU makes integration into OEM software environments easy.
Properties
- Intuitive touchscreen display
- Available in stainless steel and biocompatible PEEK
- Micro, Analytical, UHPLC, Semi-Prep, Prep and Flash Prep available
- Self-priming pump head with integrated priming/purge
- Patented floating pump seal for longer seal life
- Cartridge seal design for easy replacement via the front panel
- Solvent compressibility compensation (library of 14 solvents)
- Isocratic (Model 541) and gradient system (Model 540G)
- Integral wash pump option available
- Modbus RTU control
- OEM private label available
- Power supply: AC 100–240 V, 50/60 Hz
Technical specifications
| Pump head | Material | Flow range | Max. pressure |
|---|
| Micro | SST | 0,5 µL/min – 4 mL/min | 7.000 psi |
| Micro | PEEK | 0,5 µL/min – 4 mL/min | 4.000 psi |
| Micro UPLC | SST | 0,5 µL/min – 250 µL/min | 15.000 psi |
| Analytical | SST | 1 µL/min – 10 mL/min | 7.000 psi |
| Analytical | PEEK | 1 µL/min – 10 mL/min | 4.000 psi |
| Analytical UPLC | SST | 1 µL/min – 1 mL/min | 15.000 psi |
| Semi-Prep | SST/PEEK | 10 µL/min – 40 mL/min | 3.500 psi |
| Prep | SST/PEEK | 10 µL/min – 125 mL/min | 1.500 psi |
| Flash Chrome | SST/PEEK | 50 µL/min – 300 mL/min | 500 psi |
| Feature | Value |
|---|
| Accuracy Micro | ±1% or 1 µL/min (whichever is higher) |
| Accuracy other | ±1% or 2 µL/min (whichever is higher) |
| Precision | 0,25%, 0,10 mL/min at 20 °C |
| Pulsation | 1% ΔP/P at 1.500 psi |
| Dimensions (isocratic) | 17,1 cm B × 39,6 cm D × 17,1 cm H |
| Weight (isocratic) | 7,3 kg |
| Power supply | AC 100–240 V, 50/60 Hz, fuse 4A 250V |
